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Measuring the physical distance between two points on an image

As a requirement for a project, I need to find the walking step distance taken by a person, whose image while walking has been acquired. I have acquired a binary silhouette of the person using background subtraction and now need to calculate the actual physical distance of the step. Is there a method to convert pixel distance to physical distance in OpenCV? And if not, then what method can be applied to achieve the same?