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mean subtraction on darknet

Does it make sense to use the mean subtraction preprocessing option in blobFromImage before running a darknet DNN? I did not find any information stating that this happens at training time, so it would probably make my results worse if i run it at just inference time right?

Or is this something i should be running on my training dataset (manually) and then enable at inference time? I could not find any clear statements for this while researching online