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I'm working on a project in java, I'm using the match template. And gives me that error. anexo el codigo que tengo del metodo

public void MatchingMethod1() { Connection conect;

    Mat template = frame;
    try {
        conect = (Connection) DriverManager.getConnection("jdbc:mysql://localhost/imagen", "root", "12345");
        PreparedStatement pps = (PreparedStatement) conect.prepareStatement("SELECT imagen FROM imagenes");
        rst = pps.executeQuery();
        while (rst.next()) {

           Blob imagen = (Blob) rst.getBlob("imagen");

            Mat imagenO = Highgui.imread(imagen.toString());


            int result_cols = -imagenO.cols() + template.cols() + 1;
            int result_rows = -imagenO.rows() + template.rows() + 1;

            Mat result = new Mat(result_rows, result_cols, CvType.CV_32FC1);

            Imgproc.matchTemplate(imagenO, template, result, Imgproc.TM_SQDIFF_NORMED);
            Core.normalize(result, result, 0, 1, Core.NORM_MINMAX, -1, new Mat());
            MinMaxLocResult mmr = Core.minMaxLoc(result);
            double threashhold = 0.10;
            if (mmr.maxVal > threashhold) {

                JOptionPane.showMessageDialog(null, "Encontrado");
                break;
            } else {
                JOptionPane.showMessageDialog(null, "No coincidencias suficientes");
            }

        }
    } catch (SQLException e) {
        // TODO Auto-generated catch block
        e.printStackTrace();
    }

}

Any help is welcome