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Sorry men, i've found the solution at this question, the problem is that when you use the method get on Mat need to switch the value x and y of cordinates, otherwise could go out of range and return null value.

So must to use as follow: Mat m = something double[] buff = m.get(Y,X); //in this form if want read only few values double value=buff[0]; //contain the pixel intensity for grayscale image //or intensity of one channel according to the model RGB or BGR

However thanks alot all of you.