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I am sorry but for both 2.4 and 3.0 the support of 32 bit TIFF images is not available

• 2.4: http://docs.opencv.org/modules/highgui/doc/reading_and_writing_images_and_video.html?highlight=imwrite#cv2.imwrite
• 3.0: http://docs.opencv.org/ref/master/d4/da8/group__imgcodecs.html#gabbc7ef1aa2edfaa87772f1202d67e0ce

If you really want to save them in 32 bit, then you can store them in XML/YAML format using the FileStorage interface