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before you can assign values to your transformation Mat, you have to allocate space for those values:

    Mat par(2,3,CV_64F);

    par.at<double>(0,0)=  1.01121;  //p1
    par.at<double>(1,0)=  0.21067 ;  //p2;
    par.at<double>(0,1)= -89.69693; //p3;
    par.at<double>(1,1)= - 0.11557;  //p4;
    par.at<double>(0,2)= 1.44982;   //p5;
    par.at<double>(1,2)= -193.66149;//p6;

before you can assign values to your transformation Mat, you have to allocate space for those values:

    Mat par(2,3,CV_64F);

    par.at<double>(0,0)=  1.01121;  //p1
    par.at<double>(1,0)=  0.21067 ;  //p2;
    par.at<double>(0,1)= -89.69693; //p3;
    par.at<double>(1,1)= - 0.11557;  //p4;
    par.at<double>(0,2)= 1.44982;   //p5;
    par.at<double>(1,2)= -193.66149;//p6;