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Thank berak!

You right, but I plan to learn more about OpenCV. Certainly, OpenCV is a good means to do that.

Now I am aware that I must get knowledge of photometry since the root problem is ambient light adjustment before discretization.

Wow, It's a hard task. Maybe I can not do that only by software and may need to enter a solution with Arduino.

If someone share this same problem , we can form a team.