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unfortunately, there's no appropriate operator >> for cv::Mat, that's why reading back your txt-file fails.

you could use the FileStorage for this ( like the eigen reco saves/reads it's mats, look at the top of contrib/src/facerec.cpp ),

but i'd recommend, you store your images as images (imwrite/imread), and keep a txt-file with the filenames and label-id's

this will be much faster and take less space