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Checking nearest similarity in video using image data set

Does OpenCV support the comparison of video and image data set, returning some value (maybe a percentage) that indicates similarity? I'm trying to create an app that detects the eye glow and compare it in a data set of images of eye glows that I want to detect. I already detect the eye glow. Can I use it to compare to my data set of images of eye glows that I want to detect?