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basically, the frame you're trying to process is empty / invalid.

add some sanity checks, like:

import numpy as np
import cv2

cap = cv2.VideoCapture(0)

while(cap.isOpened()):  # check !
    # capture frame-by-frame
    ret, frame =

    if ret: # check ! (some webcam's need a "warmup")
        # our operation on frame come here
        gray = cv2.cvtColor(frame, cv2.COLOR_BGR2GRAY)

        # Display the resulting frame
        cv2.imshow('frame', gray)

    if cv2.waitKey(1) & 0xFF == ord('q'):
# When everything is done release the capture