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i: OpenCV is row major.

ii: `ImageF.at<vec3f>(y, x) == ImageF.at<vec3f>(Point(x, y));'

iii: Do not use per Pixel manipulation. For constant values of the whole image use

cv::Mat imageF = cv::Mat(nRows, nCols, nType, cv::Mat(36, 36, CV_32FC3, cv::Scalar(blue, green, red));

or

imageF.setTo(cv::Scalar(blue, green, red));

This should work

#include "opencv2/highgui/highgui.hpp"
#include <iostream>

using namespace std;
using namespace cv;

int main()
{
    Mat imageF(36,36, CV_32F);
    int i = 0;
    int j = 0;
    float blue = 0.0;
    float green= 0.0;
    float red= 0.0;
    Vec3f intensity;

    for(y=0; y<imageF.rows; y++)
        {
            for(x=0; x<imageF.cols; x++)
            {
                // The next two rows do the same
                //intensity = imageF.at<Vec3f>(y, x);
                intensity = imageF.at<Vec3f>(Point(x, y));

                //blue = intensity.val[0];
                //green = intensity.val[1];
                //red = intensity.val[2];

                blue = red = green = 0.4;
                intensity.val[0] = blue;
                intensity.val[1] = green;
                intensity.val[2] = red;

                // The next two rows do the same
                //imageF.at<Vec3f>(y, x) = intensity;
                imageF.at<Vec3f>(Point(x, y)) = intensity;
            }
        }
    imshow("Output", imageF);

    waitKey(0);
    return 0;
}

i: OpenCV is row major.

ii: `ImageF.at<vec3f>(y, x) == ImageF.at<vec3f>(Point(x, y));'

iii: You allocate an 1 channel image with CV_32F. Use CV_32FC3

iv: Do not use per Pixel manipulation. For constant values of the whole image use

cv::Mat imageF = cv::Mat(36, 36, CV_32FC3, cv::Scalar(blue, green, red));

or

imageF.setTo(cv::Scalar(blue, green, red));

This should work

#include "opencv2/highgui/highgui.hpp"
#include <iostream>

using namespace std;
using namespace cv;

int main()
{
    Mat imageF(36,36, CV_32F);
CV_32FC3);
    int i = 0;
    int j = 0;
    float blue = 0.0;
    float green= 0.0;
    float red= 0.0;
    Vec3f intensity;

    for(y=0; y<imageF.rows; y++)
        {
            for(x=0; x<imageF.cols; x++)
            {
                // The next two rows do the same
                //intensity = imageF.at<Vec3f>(y, x);
                intensity = imageF.at<Vec3f>(Point(x, y));

                //blue = intensity.val[0];
                //green = intensity.val[1];
                //red = intensity.val[2];

                blue = red = green = 0.4;
                intensity.val[0] = blue;
                intensity.val[1] = green;
                intensity.val[2] = red;

                // The next two rows do the same
                //imageF.at<Vec3f>(y, x) = intensity;
                imageF.at<Vec3f>(Point(x, y)) = intensity;
            }
        }
    imshow("Output", imageF);

    waitKey(0);
    return 0;
}

i: OpenCV is row major.

ii: `ImageF.at<vec3f>(y, ImageF.at<Vec3f>(y, x) == ImageF.at<vec3f>(Point(x, y));'ImageF.at<Vec3f>(Point(x, y));

iii: You allocate an 1 channel image with CV_32F. Use CV_32FC3

iv: Do not use per Pixel manipulation. For constant values of the whole image use

cv::Mat imageF = cv::Mat(36, 36, CV_32FC3, cv::Scalar(blue, green, red));

or

imageF.setTo(cv::Scalar(blue, green, red));

This should work

#include "opencv2/highgui/highgui.hpp"
#include <iostream>

using namespace std;
using namespace cv;

int main()
{
    Mat imageF(36,36, CV_32FC3);
    int i = 0;
    int j = 0;
    float blue = 0.0;
    float green= 0.0;
    float red= 0.0;
    Vec3f intensity;

    for(y=0; y<imageF.rows; y++)
        {
            for(x=0; x<imageF.cols; x++)
            {
                // The next two rows do the same
                //intensity = imageF.at<Vec3f>(y, x);
                intensity = imageF.at<Vec3f>(Point(x, y));

                //blue = intensity.val[0];
                //green = intensity.val[1];
                //red = intensity.val[2];

                blue = red = green = 0.4;
                intensity.val[0] = blue;
                intensity.val[1] = green;
                intensity.val[2] = red;

                // The next two rows do the same
                //imageF.at<Vec3f>(y, x) = intensity;
                imageF.at<Vec3f>(Point(x, y)) = intensity;
            }
        }
    imshow("Output", imageF);

    waitKey(0);
    return 0;
}