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you're leaking memory, in the end it can't allocate another Mat.

if you use temporary Mat's created with new, you have to release() them manually, java's gc does not see natively, c++ allocated memory (for the pixels)

Mat grayImage1 = new Mat();
Imgproc.cvtColor(frame1, grayImage1, Imgproc.COLOR_BGR2GRAY);
// ... process grayImage1 
// ... later:
grayImage1.release()