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If you want it to write it to another file, then you can use something like this inside the loop

    stringstream ssfn;
    ssfn << filenumber << ".png";
    filename = ssfn.str();
    filenumber++;

and for writing it to that file

crop  = frame(roi);
imwrite(filename,crop);

If you want to write to the same file name

try
{
    remove("fileName.ext");
}
catch(Exception e){}
crop  = frame(roi);
imwrite("filename.ext",crop);