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I hope the following code you can help (java OpenCV 2.4.10 NetBeans IDE 7.3)

int Numkadr2 = -1;
Mat Kadr1N = new Mat();
Mat Kadr2N = new Mat();
public Mat detectMotion(Mat src) {
    if (Numkadr2 == -1) {
        Kadr1N = src.clone();
        Kadr2N = src.clone();
    }
    Numkadr2++;
    if (Numkadr2 == 1) {
        Kadr1N = src.clone();
    }
    if (Numkadr2 == 2) {
        Kadr2N = src.clone();
        Numkadr2 = 0;
    }
    Point pt1 = new Point();
    Point pt2 = new Point();
    Mat first_frame = new Mat();
    Mat second_frame = new Mat();
    Imgproc.cvtColor(Kadr1N, first_frame, Imgproc.COLOR_BGR2GRAY);
    Imgproc.cvtColor(Kadr2N, second_frame, Imgproc.COLOR_BGR2GRAY);
    Mat flow = new Mat(Kadr1N.size(), CvType.CV_32FC2);
    Video.calcOpticalFlowFarneback(first_frame, second_frame, flow, 0.5, 3, 15, 3, 5, 1.1, 0);
    Mat outMat = frame.clone();

    for (int i = 0; i < Kadr1N.size().height; i += 30) {
        for (int j = 0; j < Kadr1N.size().width; j += 30) {
            pt1.x = j;
            pt1.y = i;
            pt2.x = j + flow.get(i, j)[0];
            pt2.y = i + flow.get(i, j)[1];
            Scalar color = new Scalar(255, 255, 0, 255);
            Core.line(outMat,
                    pt1,
                    pt2,
                    color, 2, 8, 0);
        }
    }
    return outMat;
}

Источник : http://me10.sblo.jp/archives/20140225-1.html