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Hi Kovand,

I'm dealing with this problem now. This is my first time looking at OpenCV, but I didn't see anything too promising. On the Wikipedia page for "Focus stacking", there is a table of software, some of which is free-ware/open-source. I found Enblend/Enfuse, which I have just started using and it works like a charm. You have to read the manual to understand the options, but it should take under a day to become a pro.

Good luck!