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Hi, you can get the ROI of the mouth by adding the following code to the second for loop

cv::Mat croped_image = frame(cv::Rect(pt1, pt2));

for example:

  for (int k = 0; k < mouthi.size(); k++) 
  {
    Point pt1(mouthi[0].x + faces[i].x, mouthi[0].y + faces[i].y);
    Point pt2(pt1.x + mouthi[0].width, pt1.y + mouthi[0].height);
    rectangle(frame, pt1, pt2, Scalar(255, 0, 0), 1, 8, 0);

    cv::Mat mouth_image = frame(cv::Rect(pt1, pt2));
    // do something with the croped mouth here
    // ...

  }