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If I understand your question correctly, you want to make a copy of the array slices, and not just have a view?
If so, replace tmp_image=frame[x*roi_height:(x+1)*roi_height, y*roi_width:(y+1)*roi_width] with tmp_image = np.copy(frame[x*roi_height:(x+1)*roi_height, y*roi_width:(y+1)*roi_width]) or something similar.
