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Remove this release and destroyAllWindowws and put at the end of code. Like this:

# Exit if ESC pressed
    k = cv2.waitKey(1) & 0xff
    if k == 27: break

cap.release()
cv2.destroyAllWindows()

Remove this release and destroyAllWindowws and put at the end of code. Like this:

# Exit if ESC pressed
    k = cv2.waitKey(1) & 0xff
    if k == 27: break

cap.release()
cv2.destroyAllWindows()

You cannot used both whle condition block. But I haven't test it yet

import numpy as np
import cv2
import sys

cap = cv2.VideoCapture('C:\Sample3.mp4')

def rescale_frame(frame, percent=30):
    width = int(frame.shape[1] * percent/ 100)
    height = int(frame.shape[0] * percent/ 100)
    dim = (width, height)
    return cv2.resize(frame, dim, interpolation =cv2.INTER_AREA)

while True:
    ret ,frame = cap.read()
    if not ret:
        break
    frame25 = rescale_frame(frame, percent=25)
    # Foot Detection
    ## Define an initial bounding box
    bbox = (100, 23, 86, 200)
    # Set up tracker.
    tracker = cv2.TrackerKCF_create()
    tracker_type = 'KCF'

    #  Uncomment the line below to select a different bounding box
    bbox = cv2.selectROI(frame25, False)
    # # Initialize tracker with first frame and bounding box
    ok = tracker.init(frame25, bbox)

    # Start timer
    timer = cv2.getTickCount()

    # Update tracker
    ok, bbox = tracker.update(frame)

    # Calculate Frames per second (FPS)
    fps = cv2.getTickFrequency() / (cv2.getTickCount() - timer);

    # Draw bounding box
    if ok:
        # Tracking success
        p1 = (int(bbox[0]), int(bbox[1]))
        p2 = (int(bbox[0] + bbox[2]), int(bbox[1] + bbox[3]))
        cv2.rectangle(frame, p1, p2, (255, 0, 0), 2, 1)
    else:
        # Tracking failure
        cv2.putText(frame, "Tracking failure detected", (100, 80), cv2.FONT_HERSHEY_SIMPLEX, 0.75, (0, 0, 255), 2)

    # Display tracker type on frame
    cv2.putText(frame, tracker_type + " Tracker", (100, 20), cv2.FONT_HERSHEY_SIMPLEX, 0.75, (50, 170, 50), 2);

    # Display FPS on frame
    cv2.putText(frame, "FPS : " + str(int(fps)), (100, 50), cv2.FONT_HERSHEY_SIMPLEX, 0.75, (50, 170, 50), 2);

    # Display result
    cv2.imshow("Tracking", frame)

    # Exit if ESC pressed
    k = cv2.waitKey(1) & 0xff
    if k == 27: break

cap.release()
cv2.destroyAllWindows()