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this here:
img = PATH_TO_SOURCE+str(folder_blueprint[0])+'-'+str(folder_blueprint[1])+'.png'
is the PATH to the image on disk, not an image (in memory) !
try like:
img_path = PATH_TO_SOURCE+str(folder_blueprint[0])+'-'+str(folder_blueprint[1])+'.png'
img = cv2.imread(img_path) # actually read it from disk