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sum() returns a Scalar with the sum of all pixels per channel
so, for a 3channel img (think a color image),
Scalar s = sum(img);
// s[0] is the sum of the 1st channel (B probably)
// s[1] is the sum of the 2nd channel (G probably)
// s[2] ...
in your case, the Mat only has one channel, so double s = sum(mat)[0] is the way to access it.