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you can easily pick any ROI of your Mat, and set that to a certain value.

example (let's set the 1st column to 1):

Mat M = Mat::zeros(5,6,CV_8U);

M(Rect(0,0,1,M.rows)) = 1;

cerr << M;

[  1,   0,   0,   0,   0,   0;
   1,   0,   0,   0,   0,   0;
   1,   0,   0,   0,   0,   0;
   1,   0,   0,   0,   0,   0;
   1,   0,   0,   0,   0,   0]