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Basically this guide tells you how thresholding works, but this is in the old C-style API.

http://8a52labs.wordpress.com/tag/thresholding-images-in-opencv/

After reading through it, to get the grasp of it, look at this for the C++ interface, which is much easier to handle.

http://docs.opencv.org/modules/imgproc/doc/miscellaneous_transformations.html?highlight=threshold#cv.Threshold