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You should use LineIterator class: http://docs.opencv.org/modules/core/doc/drawing_functions.html?highlight=lineiterator#LineIterator

E.g. if you want to get pixel values failing on that line:

LineIterator it(img, pt1, pt2, 8);
LineIterator it2 = it;
vector<Vec3b> buf(it.count);

for(int i = 0; i < it.count; i++, ++it)
    buf[i] = *(const Vec3b)*it;

You should use LineIterator class: http://docs.opencv.org/modules/core/doc/drawing_functions.html?highlight=lineiterator#LineIterator

E.g. if you want to get pixel values failing on that line:

LineIterator it(img, pt1, pt2, 8);
LineIterator it2 = it;
vector<Vec3b> buf(it.count);

for(int i = 0; i < it.count; i++, ++it)
    buf[i] = *(const Vec3b)*it;