A tricky image processing problem. (blurry moving dots)

asked 2014-06-17 13:56:25 -0600

loveandkindness gravatar image

updated 2014-06-17 14:04:08 -0600

berak gravatar image

I have a large sequence of images with dots 2-7 pixels in size moving around. The images are blurry and contain numerous unwanted blips, but the only things that are persistent and moving are the points.

I'd like to subtract everything from the image sequence except those points.

I'm new to image processing, and am not sure where my first step should be. I'm thinking equalizeHist, adaptiveThresholding, and BackgroundSubtractorMOG are a good place to start. Is this a good idea?

Is there a more formal method for this kind of thing? I've read through more than a few tutorials now, but they have all been scattered knowledge.

Thank you very much for any advise you can offer!

(I'm most comfortable with Python.)

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sample image ?

berak gravatar imageberak ( 2014-06-17 14:03:37 -0600 )edit
1

These are ecoli cells under 40x magnification. The images I will be dealing with will be at 10x (making them look like points).

http://www.rpgroup.caltech.edu/courses/aph162/2006/webpages/SizeAndRate/Oren-Nick/E._coli_40X.jpg

loveandkindness gravatar imageloveandkindness ( 2014-06-17 14:33:26 -0600 )edit
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