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use doc :

th = threshold(s,thresed,50, 255, cv2.THRESH_BINARY_INV);

use doc :

th = threshold(s,thresed,50, threshold(s,threshed,50, 255, cv2.THRESH_BINARY_INV);

use doc :

th = threshold(s,threshed,50, 255, cv2.THRESH_BINARY_INV);
THRESH_BINARY_INV);